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dc.creatorFantinel, Mara Rejane
dc.date.accessioned2012-11-12
dc.date.available2012-11-12
dc.date.issued2012-02-14
dc.identifier.citationFANTINEL, Mara Rejane. EFFECT OF Paullinia cupana in vitro oxidative metabolism HUMAN SPERM EXPOSED TO CHLORIDE METHYLMERCURY. 2012. 89 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012.por
dc.identifier.urihttp://repositorio.ufsm.br/handle/1/11188
dc.description.abstractInvestigations suggests that methylmercury (MeHg) toxicitiy is related to oxidative stress promotion caused by lipid peroxidation of cell membranes as well as genotoxicity. The methylmercury (MeHg) exposition causes production of adverse effects as deterioration of semen quality, testicular degeneration, and male reproductive failure. Since human sperm is a vulnerable cell investigations analyzing the antioxidant protection of plant extracts on MeHg exposition is relevant. Among Brazilian food supplements we found guaraná (Paullinia cupana, Mart.) which is rich in caffeine, other metaxanthynes and catechins. Previous studies showed that guaraná present antioxidant, antimutagenic, anticarcinogenic properties that could exert protective effect of sperm cells exposed to MeHg. Therefore, an in vitro protective role of guaraná (Paullinia cupana, Mart.) viability and oxidative stress modulation of human sperm cells exposed to methylmercury intoxication was here in investigated. The human sperm cell collected from donators were counted, aliquoted and submitted to four treatments negative control (C), MeHg exposition (MeHg, 60 μM); (3) Guaraná supplementation (Gua, 10 mg/mL) and MeHg exposition plus guaraná suplementation (MeHg-Gua). During the treatment exposition the samples were maintained at 37oC at 95% CO2 incubation. The viability of spermatozoa was analyzed by MTT reduction assay. Lipoperoxidation was estimated by determination of the thiobarbituric acid derivative of MDA (TBA-MDA) using a sensitive highperformance liquid chromatographic (HPLC), reactive oxygen species (ROS) was verified by testing with fluorimetric 2′ 7′-dichlorofluorescein diacetate (DCFH-DA) assay. Protein carbonylation and thiol were evaluated spectrophotometrically. The guaraná treatment increase the sperm viability exposed to MeHg as well as decreased the lipoperoxidation, ROS and protein carbonylation levels. Thus, we conclude that the guaraná showed in vitro protective effect of human sperm against MeHg exposition.eng
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.formatapplication/pdfpor
dc.languageporpor
dc.publisherUniversidade Federal de Santa Mariapor
dc.rightsAcesso Abertopor
dc.subjectMercúriopor
dc.subjectToxicidadepor
dc.subjectGuaranápor
dc.subjectEspermatozoidespor
dc.subjectInfertilidadepor
dc.subjectMercuryeng
dc.subjectToxicityeng
dc.subjectGuaranaeng
dc.subjectSpermeng
dc.subjectInfertilityeng
dc.titleEFEITO in vitro DE Paullinia cupana NO METABOLISMO OXIDATIVO DE ESPERMATOZÓIDES HUMANOS EXPOSTOS AO CLORETO DE METILMERCÚRIOpor
dc.title.alternativeEFFECT OF Paullinia cupana in vitro oxidative metabolism HUMAN SPERM EXPOSED TO CHLORIDE METHYLMERCURYeng
dc.typeDissertaçãopor
dc.description.resumoInvestigations suggests that methylmercury (MeHg) toxicitiy is related to oxidative stress promotion caused by lipid peroxidation of cell membranes as well as genotoxicity. The methylmercury (MeHg) exposition causes production of adverse effects as deterioration of semen quality, testicular degeneration, and male reproductive failure. Since human sperm is a vulnerable cell investigations analyzing the antioxidant protection of plant extracts on MeHg exposition is relevant. Among Brazilian food supplements we found guaraná (Paullinia cupana, Mart.) which is rich in caffeine, other metaxanthynes and catechins. Previous studies showed that guaraná present antioxidant, antimutagenic, anticarcinogenic properties that could exert protective effect of sperm cells exposed to MeHg. Therefore, an in vitro protective role of guaraná (Paullinia cupana, Mart.) viability and oxidative stress modulation of human sperm cells exposed to methylmercury intoxication was here in investigated. The human sperm cell collected from donators were counted, aliquoted and submitted to four treatments negative control (C), MeHg exposition (MeHg, 60 μM); (3) Guaraná supplementation (Gua, 10 mg/mL) and MeHg exposition plus guaraná suplementation (MeHg-Gua). During the treatment exposition the samples were maintained at 37oC at 95% CO2 incubation. The viability of spermatozoa was analyzed by MTT reduction assay. Lipoperoxidation was estimated by determination of the thiobarbituric acid derivative of MDA (TBA-MDA) using a sensitive highperformance liquid chromatographic (HPLC), reactive oxygen species (ROS) was verified by testing with fluorimetric 2′ 7′-dichlorofluorescein diacetate (DCFH-DA) assay. Protein carbonylation and thiol were evaluated spectrophotometrically. The guaraná treatment increase the sperm viability exposed to MeHg as well as decreased the lipoperoxidation, ROS and protein carbonylation levels. Thus, we conclude that the guaraná showed in vitro protective effect of human sperm against MeHg exposition.por
dc.contributor.advisor1Cruz, Ivana Beatrice Mânica da
dc.contributor.advisor1Latteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790333D6por
dc.contributor.referee1Rocha, Maria Izabel de Ugalde Marques da
dc.contributor.referee1Latteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4700142P8por
dc.contributor.referee2Leal, Daniela Bitencourt Rosa
dc.contributor.referee2Latteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706487E7por
dc.creator.Latteshttp://lattes.cnpq.br/5570364071621144por
dc.publisher.countryBRpor
dc.publisher.departmentBioquímicapor
dc.publisher.initialsUFSMpor
dc.publisher.programPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológicapor
dc.subject.cnpqCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICApor


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