Estabelecimento de protocolo para desinfestação superficial de segmentos nodais de Olea europaea L.

Abstract

Considering the relevant economic and pharmaceutical importance of the olive tree (Olea europaea L.), the species has been widely cultivated in several countries around the world. In this way, the search for propagation methods that provide greater productivity in a shorter time has been the objective of current research. Micropropagation presents itself as a viable alternative due to characteristics such as increased genetic quality and relatively fast gains in productivity. However, there are many difficulties in the process of superficial disinfestation of nodal segments, used as explants, which significantly affect the efficiency of this cloning technique. In this way, the establishment of a protocol to promote the disinfestation of nodal segments of two olive cultivars (Arbequina and Picual) through experiments with different concentrations of sodium hypochlorite, lysoform and cyclozyme at different immersion times and submission of the explants to electrical discharges was the objective of this work. For this, an entirely randomized design (DIC) was used with different numbers of treatments and repetitions for each experiment. No effective results were found for the electrical discharges + standard disinfestation treatment in the decontamination of explants, however, it is suggested that the voltage used or the time of exposure to discharges were not sufficient to promote disinfestation. At the end of the evaluations, the effectiveness of the application of disinfectants was verified, with the highest rate of axenic explants (70%) being obtained in the NaOCl + lyzoform + ciclozyme treatment together for 10 minutes and with explants from the clonal garden. However, this treatment was not responsible for the highest rate of responsiveness of the explants, being the treatment with NaOCl + lyzoform + ciclozyme with explants from the same source for the period of 2 minutes responsible for the highest rate of 23.3%.