Indução pós-colheita da síntese de resveratrol e de resistência de frutos a podridões

Abstract

The objectives of this work were divided in two experiments phases. In the first phase, it was studied the presence of trans-resveratrol in temperate climate fruits and the variation of their concentration in these fruits during storage period, as well the amount of total phenols and total anthocyanins. It was evaluated total phenols, total anthocyanins and trans-resveratrol, before and after cold storage and under controlled atmosphere; and after five days shelf-life at 20°C in Bruno kiwifruit; Araza and Yvahe strawberries; Black mulberry; Galaxi , Gala and Fuji apples; persimmon Fuyu ; Bleugem blueberry, Isabel , Merlot and Niagara Pink grapes. It was detected trans-resveratrol in natura in flesh Bruno kiwifruit; Araza and Yvahe strawberries; Black mulberry. In other fruits, it was detected transresveratrol in natura in the skin. The cold storage stimulated trans-resveratrol synthesis in Bleugem blueberry, and Isabel and Merlot grapes. In the second phase, it was evaluated the induction of trans-resveratrol synthesis and its possible action as phytoalexin in Gala and Fuji apples stored in controlled atmosphere were evaluated. Experiments were with the application of abiotic elicitors: ultraviolet irradiation, phosphite and acibenzolar-S-methyl, applied before controlled atmosphere storage, and intermittent ozone applied during storage of to Gala and Fuji apples. Rot was controlled in another experiment with the same elicitors. In fruit bark it was analyzed trans-resveratrol; total phenols and total anthocyanins, as well as the diameter of injury after inoculation of Penicillium sp. The abiotic elicitors were induced in 'Fuji' apples trans-resveratrol synthesis in the following sequence: Acibenzolar-Smethyl > phosphite ³ UV-C irradiation ³ ozone, but it wasn t effective on 'Gala' apple. There was no correlation between the syntheses of trans-resveratrol and rotting control, with exception of phosphite was control of rot in 'Gala'.