Avaliação de alternativa de extração e quantificação de tricotecenos em amostras de milho utilizando cromatografia líquida acoplada à espectrometria de massas em Tandem

Abstract

Mycotoxins are a global problem that impacts food and feed safety and causes significant economic losses. The trichothecenes are a group of more than 200 chemically different mycotoxins, produced mainly by Fusarium spp. fungi, and are divided into four groups, with type A and B trichothecenes being the most relevant from a health and economic point of view. The chemical differences between the trichothecenes make analytical methodologies that include all of them difficult to elaborate, requiring complex procedures to extract all the desired mycotoxins, using solid phase extraction for example, making the procedure more costly and time consuming. The objective of the present study was to evaluate an alternative methodology capable of recovering seven trichothecenes, being T-2, HT-2 and DAS of type A and 3-AcDON, 15-AcDON, NIV and FusX of type B, in corn samples fortified with commercial standards in 3 concentrations (80, 300 and 600 μg/L) through liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS). The preliminary results obtained in this study successfully recovered six of the seven trichothecenes tested, bringing positive prospects that this methodology can be used to aid in the assessment of trichothecene levels more quickly and cheaply than currently available methodologies.