Atividade de diferentes formulações de Melaleuca alternifolia e terpinen-4-ol em isolados de Rhodococcus equi de diferentes origens

Abstract

The aim of this study was to determine the bactericidal and bacteriostatic activity of a medicinal plant, Melaleuca alternifolia, in different formulation: oil free , essential oil associated with nanocarriers (nanoemulsion and nanocapsule), besides the associations between free oil and nanoformulations, as well as the antimicrobial activity of its major compound, terpinen - 4 - ol , in Rodococcus equi isolates (n = 24 ) from different sources. In order to determine the minimal inhibitory (MIC) and the minimum bactericidal (MBC) concentrations the microdilution method in Mueller-Hinton broth was employed in this study. The values of MIC and MBC ranged from 312.5 mg/mL to 2.500μg/mL. The results obtained with essential oil of M. alternifolia demonstrated low activity in oil free presentation; however, when it was associated with nanocarriers, its antimicrobial activity was enhanced, showing bacteriostatic activity. It was possible to verify that against R. equi isolates, both associations as well the major compound tested, showed both bactericidal and bacteriostatic activities. It is suggested that M. alternifolia, in association with nanocarriers systems, as well as terpinen -4-ol, presents potential for future studies concerning the equine rodococosis therapy.