Avaliação dos efeitos biológicos do (s) (3-feniltelanil propanamida) succinato de dimetila, um novo telúrio aminoácido derivado do ácido aspártico

Abstract

Recent studies from our group demonstrated that the (S)-dimethyl 2-(3-(phenyltellanyl) propanamido) succinate compound, a telluroamino acid showed glutathione peroxidase (GPx)-like activity, attesting to its antioxidant potential. However, toxicology and pharmacology from this compound are unknown. Then, the present study aimed to investigate the potencial toxicological properties of this compound in vitro and in vivo. For in vitro analysis were used biochemical tests as: GPx-like activity, tiol oxidase activity and TBARS determination. Futhermore, the in vivo analyses were carried out using swiss albino male adult mice treated and submitted to behavioral tests (exploration and locomotion). For ex vivo studies, the antioxidant enzyme activity, and tests to evaluate citoxicity, genotoxicty and mutagenicity of compound were carried out. In order to verify the potencial mechanisms of action of the compound, we used the GR and TrxR enzymes inhibitors. The results indicated that the compound presented an important antioxidant activity in vitro. However, when the compound was tested in vivo with the highest concentrations, it was shown as a potent genotoxic and mutagenic agent at doses of 175 e 195 μmol/Kg. On the other hand, when the compound was tested at minor doses (1/2 LD50), it did not demonstrate significant signs of toxicity. In relation to treatment with inhibitors of GR and TrxR enzymes, the results showed that the inhibition of these enzymes did not affect the compound action in our model of study.