Efeitos dos recursos terapêuticos sobre marcadores bioquímicos na lesão muscular por estiramento em ratos

Abstract

The sports practice can induce injuries in the muscular fibers, generating an oxidative damage and inflammatory process. Therapeutic practices are used to reduce these damages, such as platelet-rich plasma (PRP) and cold water immersion (CWI). Thus, the present study aims to investigate through biomarkers the influence of treatment with CWI and / or PRP in muscle stretch model by stretching in rats. Methods: Adult male Wistar rats were subjected to stretch muscle injury and immediately, the animals received an intramuscular application of PRP and / or subjected to CWI. Biochemical analyzes such as: thiobarbituric acid reactive species (TBARS), dichlorofluorescine reactive species (DCFRS), thio groups (-SH), reduction of MTT, reduced glutathione (GSH), oxidized glutamine (GSSG), superoxide dismutase (SOD) , catalase (CAT), myeloperoxidase (MPO), were performed 1, 3, 5, 7 days after the first day of treatment. Stretch lesion in the gastrocnemius of the animals caused an increase in the markers of oxidative damage, such as the formation of reactive species of dichlorofluorescein and lipid peroxidation in muscle and blood. Combinad of PRP and CWI treatment appears to be the most effective in preventing muscle injury, compared to treatments isolated, possibly due to its ability to modulate damage to muscle cell structure and also the intensity of the inflammatory response following skeletal muscle injury.