Ação da via de sinalização Hippo durante o reconhecimento materno da gestação em ovelhas após balanço energético negativo induzido

Abstract

The initial period of pregnancy is very critical for the occurrence of early embryonic losses and failures in maternal-fetal communication and often occurs under a negative energy balance (NEB), which can affect negatively during this period. The Hippo signaling pathway is highly conserved between species, especially during embryogenesis, and is part of the embryonic control of Interferon tau (IFNT). Our study uses a NEB model induced during early pregnancy in ewes to evaluate the effects on the Hippo signaling pathway. For this, 21 ewes were allocated to four groups: Non-pregnant control (PN); pregnant control (P); NEB during the first week of pregnancy (FW); and NEB during the second week of pregnancy (SW). The group NP was greater mRNA for BIRC5 and CYR61 in the corpus luteum (CL) (P <0.05). The expression of CYR61 was different between the FW and SW groups in the CL (P <0.05). The SW group had a greater concentration of progesterone (P <0.05) than other experimental groups. Our results obtained that NEB does not alter the initial embryonic signaling. Metabolic stress has a major influence on the circulating concentration of progesterone. Also, the Hippo signaling pathway appears to be inactive in the endometrium during early pregnancy, due to the large endometrial proliferation on Day 17. However, in CL the Hippo signaling pathway is inactive during the estrous cycle, probably due to apoptosis. However, in pregnant animals, the Hippo signaling pathway is active in the CL, which means that progesterone tuning can be independent of the Hippo signaling pathway.