Desenvolvimento de método para determinação de resíduos de agrotóxicos em azeite de oliva empregando GC-MS/MS

Abstract

In Brazil, olive oil is defined as a product obtained from the fruits of the olive tree Oleaeuropaea L. and it is already configured in the international scenario as the second largest importer of olive oil. Olive oil has been used in cooking for thousands of years. Rio Grande do Sul is the state with the highest production of olives. With the intention of improving the cultivation and the quality of the final product, it is customary to use pesticides. In Brazil, the current legislation is only for olives with an MRL of 9 pesticides. Thus, the uses of pesticides that are not allowed end up being used. Therefore, to guarantee the quality of olive oils, developing an analytical method that allows the determination of pesticides in olive oil is essential. Thus, this research developed and validated an analytical method for the determination of 79 different pesticides in olive oil, using gas chromatography coupled to tandem mass spectrometry (GC-MS/MS). The method was validated for 79 compounds, and the limit of quantification (LOQ) ranged from 25 to 100 μg kg-1 and the limit of detection (LOD) from 7.5 to 30 μg kg-1 within the acceptance range used. which was 70 to 120% with RSD <20%. The method was applied to 18 samples of olive oil. Of these, 7 olive oil samples showed pesticide residues of different classes and characteristics at concentrations ranging from 8.91 to 689.41 μg kg-1. Considering the results obtained, the proposed method proved to be efficient in the determination of 79 pesticides in olive oil.