Verbena litoralis Kunth (Verbenaceae) micropropagação, estaquia e análise de flavonóides e cumarinas

Abstract

The species Verbena litoralis Kunth is popularly known as wort or father-caetano gervãozinho field, and southern Brazil is used as detoxifying, antipyretic and diarrhea. The aim of this study was to vegetative propagation through cuttings and micropropagation of V. litoralis, evaluate the physiological parameter settings as chlorophyll fluorescence, photosynthetic pigments and identify the phytochemical compounds present in the extracts of plants obtained in vitro after acclimatization of the same. The experiment was conducted using micropropagation complete MS plus 0.1 ml L-1 fungicide (carbendazim®), 30 g L-1 sucrose, 6.0 g L-1 agar and four combinations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA): 0, 1.0, 2.0, 4.0 and 0, 0.1, 0.2, 0.4 mg L-1, respectively, and presence or absence of coal enabled (1 mg L-1). The plants obtained in vitro were transferred to disposable cups and kept in a growth chamber throughout the experiment with a temperature of 25 ± 2 º C and 16 hours photoperiod. The phytochemical analysis of the extract of the aerial parts and roots of V. litoralis, obtained by maceration hydroethanol was performed by HPLC and detected percentages of near 4.76% rutin, quercetin 9.18% and 0.031% coumarin. In micropropagation, the absence of BAP and NAA led to a percentage of 21% of grown plants. The acclimatization showed homogeneous development since all were subjected to the same substrate type and temperature.