Efeito do treinamento hipóxico intermitente sobre o ganho de peso, consumo calórico e perfil bioquímico em ratos submetidos à diferentes dietas

Abstract

This work investigated the effect of intermittent hypoxic training (IHT) sessions under bodyweight, food intake and biochemical parameters in Wistar rats fed normo fat (NF) and high fat (HF) diets. Forty rats were divided in two groups, which were fed a NF or a HF diet. Afterwards the rats were subdivided in four groups and submitted to intermittent hypoxia (IH) or normoxia (N) sessions for 30 days. Groups formed: NF/N, NF/IH, HF/N and HF/IH. The groups submitted to IHT followed this protocol: 15 minutes hypoxia, 10-15% inspired O2 and 5 minutes for reoxigenation). Sham group was exposed to the same conditions but with normal O2 concentrations (21%). Bodyweight and food intake were measured every two days. The rats were anesthetized on day 31 and the blood was sampled by cardiac punction for analyses. The biochemical parameters analyzed were: total cholesterol (TC), triglycerides (TG), high density cholesterol (HDL), glucose, lactate dehidrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and muscle and hepatic glycogen. In regard to diets, the HF group promoted the biggest weight gain just in normoxia groups.The food intake decrease significantly in HF/IH subgroup compared to HF/N. Although the average of the corporal weight did not decrease significantly, the differences in percentages had shown a reduction in HF/IH subgroup. In the profile biochemist, the IHT only modified the LDH, increasing it in NF subgroup and the AST, diminishing it in HF subgroup. Our findings allow to suggest that the IHT can have a promising role in the prevention of the obesity and more studies are needed to evaluate its effect in the prevention/treatment of other diseases.