The aim of this study was to analyze the effect of an intermittent exercise session and an anaerobic exercise session on blood cell counts and oxidative stress in middle-aged women. A number of 26 middle aged women were divided in three groups: RE group (which was submitted to a 60 min of resistance exercises), Spinning Group (which was submitted to a 60 min of Spinning) and control group (which remained at rest). Sample collection was carried out at rest, immediately after, and 1 hour after exercise sessions. The blood count was performed by differential count of leukocytes. Oxidative stress was assessed by determining the lipid peroxidation and protein carbonylation as well as the measurement of enzymatic and non-enzymatic antioxidant body defenses. In both groups lymphocytes and monocytes had a decrease after 1 h recuperation when compared to after exercise. TBARS levels showed an increase in lipid peroxidation after exercise. Protein carbonyl had a significant increase after exercise. SOD and CAT activities had a decrease in both groups immediately after exercise. In conclusion, we found that an acute bout of intermittent and anaerobic exercises induces immune suppression and increases the production of reactive oxygen species, causing oxidative stress in middle aged women.