Atividade antioxidante e antimicrobiana de extratos de ora-pro-nobis (Pereskia aculeata Mill.) e sua aplicação em mortadela

Abstract

Mortadella is one of the meat products with good nutritional value and excellent consumer acceptance. In addition to protein and lipid source, a plethora of non-meat ingredients have been used in the preparation of emulsified products. The use of natural compounds to increase the shelf life of meat products is a promising alternative, since many plant substances possess antioxidant and antimicrobial properties. The ora-pro-nobis is considered a nutritional supplement because of its protein content, fiber, iron, calcium, among others. This study aimed to obtain extracts from the leaves of ora-pro-nobis and evaluate the antioxidant and antimicrobial activity, in addition, apply the extract with antioxidant activity in bologna, aiming as natural antioxidant potential. First, it applied the chemical and mineral composition of the leaves of ora-pro-nobis. Extracts were obtained by conventional stirring, varying the solvent (water and ethanol), time (1 and 24h) and temperature (95 °C and 25 °C). In the extracts were carried out analysis of total phenolics, total flavonoids, antimicrobial and antioxidant activity in vitro through the DPPH methods, FRAP and ABTS radical ● +. The extract obtained by extraction by shaking using distilled water as a solvent and 95 °C showed the best antioxidant properties, so it has been applied in different concentrations in bologna. After the finished product were carried out physico-chemical, sensory and microbiological analysis. The bologna presented within the requirements of legislation for both the chemical composition and for microbiological stability. They showed no oxidative changes during storage. He observed a tendency of increasing the pH of all treatments during the storage time. The mortadella added 0.2% sodium erythorbate and 2.0% extract showed the lowest lipid oxidation after 70 days of storage. The sensory evaluation showed a good acceptance by consumers. It can be concluded that the extract from the leaves of ora-pro-nobis presented with antioxidant potential to be tapped by carnea industry.