Alotransplante parcial de vesícula urinária com células mesenquimais estromais multipotentes alogênicas derivadas do tecido adiposo em coelhos

Abstract

The study was divided in three articles. The last two were part of the same experiment, which was divided for better explanation and details of the procedures performed and parameters evaluated. The first described the features and current uses of adult MSCs derived from the bone marrow, adipose tissue and dental pulp, as well as laboratory protocols and in vivo experiments developed in the Laboratory of Experimental Surgery of the Federal University of Santa Maria aiming at application of these cells in veterinary patients. The second aimed to characterize the stones and urinalysis of domestic rabbits underwent allograft urinary bladder. For this 25 New Zealand White rabbits, which underwent partial allograft bladder, being treated with cyclosporine (GCi) or mesenchymal stem cells (GCe). In this study, 58.3% (7/12) of animals GCi had bladder stones, however, only 33.3% (4/12) rabbits GCe showed bladder urolithiasis. It was concluded that there were fewer animals (33.3%) with bladder calculus in the group treated with MSCs, calcium being the most prevalent mineral in both groups. Urinalysis showed that MSC-treated animals showed no hematuria and / or bacteriuria. The third article aimed to evaluate clinical, sonographic and anatomophysiological partial bladder fresh allograft in rabbits (Oryctolagus cuniculus), using as immunomodulator allogeneic MSCs derived from adipose tissue. We used 25 rabbits, one being male and the donor of MSCs, and the other 24 females who underwent partial allograft bladder, being treated with cyclosporine (GCi) or mesenchymal stem cells (GCe). The treatments used in the study reached satisfactory clinical results, preventing allograft rejection. All animals had satisfactory recovery, showing no clinical or behavioral change as a result of treatment throughout the period of post-surgical evaluation. It is concluded that ADSCs, in a single application, were sufficient to prevent clinical and ultrasound signs of rejection from allograft of the urinary bladder, which maintained the anatomophysiological structure for 30 days in rabbits.