Variação na desidratação das hemácias e sua correlação com o RDW

Abstract

When hematias are dehidrated “in vitro” with a hipertonic NaCl solution, under an ideal concentration (with no risk of hemolisis), it’s possible to purpose on solid basis that hidratation levels increase gradually, and are proportional to their hemoglobinic insaturated state (desceased of HCM), even when it is not yet detectable by the routine methods. Methodology: A 32 hemograms group was formed, for wich are required a different levels of hipochromic anemy, and the superior limit of 12 g/dl hemoglobin concentration. Those samples were analised in a Coulter Counter STKS, and from their hemograms are selected the RDWs those would be used like a reference group. Each one of these samples was submited to the dehidratation process, as purposed in this article as a pilot project. Finally, the results of 540 nanometers spectrophotometer reading from 1:400 dilucting suspensions of hematias, was compaired with their respectives RDWs. 72,3% from anemic patients who showed in the comparative readings of transmittance over an 8 % direct diference between isotonic and hipertonic dilutions of hematias, also presented anisocitosis compatible RDWs (over 15 % limit). The independence test was made with the results, and the exact probability of the test obtained was p =0,0141. Conclusion: There is a significative dependence relation between RDWs and hematias hidratation increases.