Implante ósseo cortical alógeno conservado em mel na reconstrução de falha óssea diafisária em fêmur de cães

Abstract

Fourteen adult mongrel dogs were used to evaluate the honey preserved cortical allografts in the repair of diaphyseal femoral defect. The allografts were inserted into a 5cm segmental defect created in the mid-diaphysis of the right femur in each dog. The bones were stabilized with a 3.5mm dynamic compression plate and eight 3.5mm bone screws. Each dog was examined daily to evaluate complications and weight-bearing of the operated limb. Radiographs of the right femur were taken postsurgically and once every 15 days for the next three months. Following this, radiographs were taken once per month until the end of the research. Each two animals were euthanized after 30, 60, 90, 120, 180, 270, and 360 days. The right femur was harvested and prepared for histological evaluation. Nineteen (79.17%) of the twenty-four host-graft interfaces were radiographically healing (union). The mean time to allograft incorporation was 67.10 days (range, 45-90 days). There was no statistical difference in the allograft incorporation time between proximal and distal host-graft interfaces. It was observed Bacillus contamination in three honey samples, however, in allografts it was not verified. Good incorporation of donor graft by bone was observed histologically. Initially, there were osteoclastic activity increases in graft surfaces, and after bone formation. Complications observed were nonunion, allograft fracture, and allograft resorption. We conclude that despite the complications, honey preserved cortical allografts are a viable options to bone reconstruction.