dc.creator | Traesel, Carolina Kist | |
dc.date.accessioned | 2017-05-30 | |
dc.date.available | 2017-05-30 | |
dc.date.issued | 2013-03-08 | |
dc.identifier.citation | TRAESEL, Carolina Kist. Genotypic analysis and phylogeny based on glycoproteins C and D genes of bovine herpesviruses. 2013. 80 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2013. | por |
dc.identifier.uri | http://repositorio.ufsm.br/handle/1/4095 | |
dc.description.abstract | Bovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) are genetic and antigenic closely related pathogens of cattle, historically associated with respiratory/genital and neurological disease, respectively. This thesis reports the genomic and phylogenetic analyzes based on glycoproteins C (gC) and D (gD) genes of BoHV-1 and BoHV-5. Initially, the differentiation between BoHV-1 and BoHV-5 was performed by a differential PCR based on 5 gC gene. Then, a molecular analysis based on the 3 region of the gC gene of 45 BoHV isolates from Brazil, Uruguay and Argentina (1981-2009) was described. The phylogenetic tree reconstruction provided a clear distinction between BoHV-1 and BoHV-5, and BoHV-1 into subtypes BoHV-1.1 and BoHV-1.2. The levels of nucleotide (nt) similarity ranged from 99.1 to 100% among BoHV-1 sequences (n=12); 96.2-100% among BoHV-5 sequences (n=32); and 77.7-90.3% between BoHV-1 and BoHV-5 sequences. A transmembrane domain of 24 amino-acid (aa) and the putative cytoplasmic tail of 8 aa were also identified. In addition, a phylogenetic study was performed to investigate genetic divergences at the 3 region of gD gene of respiratory/genital BoHV-1 (n=7), neurological BoHV-1 (n=7) and neurological BoHV-5 (n=7) isolates, and whether these differences would be associated with the respective neurological presentation. The phylogenetic reconstruction allowed a clear differentiation of BoHV-1 (n=14) and BoHV-5 (n=7), but BoHV-1 isolates from neurological disease grouped within BoHV-1 branch. The nt and aa similarity levels were on average 98.3% among BoHV-1; 97.8% and 95.8% among BoHV-5; 73.7% and 64.1% between viral species. These results indicate that both genes revealed a high conserved 3 region within each species and a less conserved region between BoHV-1 and BoHV-5. The phylogenetic analyzes allowed differentiation of BoHV-1 and BoHV-5 species, and even subtypes grouped in distinct branches in the 3 gC gene-based study, indicating that this region represents a better choice for phylogenetic subgrouping. So, it was concluded that these genome regions represent a suitable target for phylogenetic classification of BoHV-1 and BoHV-5 isolates, and, perhaps, for understanding evolutionary relationships. However, no conclusion of a possible association of genetic differences with phenotypes could be drawn. | eng |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico | |
dc.format | application/pdf | por |
dc.language | por | por |
dc.publisher | Universidade Federal de Santa Maria | por |
dc.rights | Acesso Aberto | por |
dc.subject | BoHV-1 | por |
dc.subject | BoHV-5 | por |
dc.subject | GC | por |
dc.subject | GD | por |
dc.subject | Filogenia | por |
dc.subject | Phylogeny | eng |
dc.title | Análise genotípica e filogenética com base nos genes das glicoproteínas C e D de herpesvírus bovino 1 e 5 | por |
dc.title.alternative | Genotypic analysis and phylogeny based on glycoproteins C and D genes of bovine herpesviruses | eng |
dc.type | Tese | por |
dc.description.resumo | Bovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) are genetic and antigenic closely related pathogens of cattle, historically associated with respiratory/genital and neurological disease, respectively. This thesis reports the genomic and phylogenetic analyzes based on glycoproteins C (gC) and D (gD) genes of BoHV-1 and BoHV-5. Initially, the differentiation between BoHV-1 and BoHV-5 was performed by a differential PCR based on 5 gC gene. Then, a molecular analysis based on the 3 region of the gC gene of 45 BoHV isolates from Brazil, Uruguay and Argentina (1981-2009) was described. The phylogenetic tree reconstruction provided a clear distinction between BoHV-1 and BoHV-5, and BoHV-1 into subtypes BoHV-1.1 and BoHV-1.2. The levels of nucleotide (nt) similarity ranged from 99.1 to 100% among BoHV-1 sequences (n=12); 96.2-100% among BoHV-5 sequences (n=32); and 77.7-90.3% between BoHV-1 and BoHV-5 sequences. A transmembrane domain of 24 amino-acid (aa) and the putative cytoplasmic tail of 8 aa were also identified. In addition, a phylogenetic study was performed to investigate genetic divergences at the 3 region of gD gene of respiratory/genital BoHV-1 (n=7), neurological BoHV-1 (n=7) and neurological BoHV-5 (n=7) isolates, and whether these differences would be associated with the respective neurological presentation. The phylogenetic reconstruction allowed a clear differentiation of BoHV-1 (n=14) and BoHV-5 (n=7), but BoHV-1 isolates from neurological disease grouped within BoHV-1 branch. The nt and aa similarity levels were on average 98.3% among BoHV-1; 97.8% and 95.8% among BoHV-5; 73.7% and 64.1% between viral species. These results indicate that both genes revealed a high conserved 3 region within each species and a less conserved region between BoHV-1 and BoHV-5. The phylogenetic analyzes allowed differentiation of BoHV-1 and BoHV-5 species, and even subtypes grouped in distinct branches in the 3 gC gene-based study, indicating that this region represents a better choice for phylogenetic subgrouping. So, it was concluded that these genome regions represent a suitable target for phylogenetic classification of BoHV-1 and BoHV-5 isolates, and, perhaps, for understanding evolutionary relationships. However, no conclusion of a possible association of genetic differences with phenotypes could be drawn. | por |
dc.contributor.advisor1 | Flores, Eduardo Furtado | |
dc.contributor.advisor1Lattes | http://lattes.cnpq.br/0446078331070694 | por |
dc.contributor.advisor-co1 | Weiblen, Rudi | |
dc.contributor.advisor-co1Lattes | http://lattes.cnpq.br/7946350215388090 | por |
dc.contributor.referee1 | Kreutz, Luiz Carlos | |
dc.contributor.referee1Lattes | http://lattes.cnpq.br/6207090944092820 | por |
dc.contributor.referee2 | Spilki, Fernando Rosado | |
dc.contributor.referee2Lattes | http://lattes.cnpq.br/3481593940960227 | por |
dc.contributor.referee3 | Brum, Mário Celso Sperotto | |
dc.contributor.referee3Lattes | http://lattes.cnpq.br/9761857774819478 | por |
dc.creator.Lattes | http://lattes.cnpq.br/1887646413957746 | por |
dc.publisher.country | BR | por |
dc.publisher.department | Medicina Veterinária | por |
dc.publisher.initials | UFSM | por |
dc.publisher.program | Programa de Pós-Graduação em Medicina Veterinária | por |
dc.subject.cnpq | CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA | por |